lDDT scores were then computed using both the full target structures and, in a weight-averaged AU-based form, for a range of Ro, values from 1 to 40 . There are many ways to lessen the amount of radon in a home to a safe level. However, because protein structures are rich in rigid structural elements like -helices and -sheets, where the relative local positions are restricted, they show in general a higher number of preserved local distances than random polymers. Choose from hundreds of free courses or pay to earn a Course or Specialization Certificate. GS and MM exhibit a very significant correlation, implying that hub genes of the brown module also tend to be highly correlated with weight. Here we briefly outline the main functionality of the package and highlight new contributions. MOTIF DISCOVERY. A fourth analysis goal is to annotate all network nodes with respect to how close they are to the identified modules. Hence, the weighted GDC-all scores can be considered to be largely devoid of the influence of domain movements. as the i-th node profile across m sample measurements. Random assignment does not guarantee that the groups are matched or equivalent. Peirce's experiment inspired other researchers in psychology and education, which developed a research tradition of randomized experiments in laboratories and specialized textbooks in the eighteen-hundreds.[3][4][5][6]. i In the heatmap, green color represents low adjacency (negative correlation), while red represents high adjacency (positive correlation). Olechnovic K, et al. One interesting feature in Figure 4 is the presence of several peaks at larger threading errors (e.g. turquoise module genes form a reddish square in the TOM plot. To enhance the integration of WGCNA results with other network visualization packages and gene ontology analysis software, we have created several R functions and corresponding tutorials. Genetics 2008, 179(2):10891100. The code is organized into short sections, each of which addresses a particular task. Jerzy Neyman advocated randomization in survey sampling (1934) and in experiments (1923). If a test of statistical significance is applied to randomly assigned groups to test the difference between sample means against the null hypothesis that they are equal to the same population mean (i.e., population mean of differences = 0), given the probability distribution, the null hypothesis will sometimes be "rejected," that is, deemed not plausible. Network visualization plots. An alternative is a multi-dimensional scaling plot; an example is presented in Figure 2B. WGCNA: an R package for weighted correlation network analysis, http://www.genetics.ucla.edu/labs/horvath/CoexpressionNetwork/Rpackages/WGCNA, http://www.image.ucar.edu/GSP/Software/Fields, http://creativecommons.org/licenses/by/2.0. The network and the 18 identified modules are depicted in Figures 4A, B. However, owing to the choice of different templates, models often have a higher similarity to one or the other reference structure, and the choice of reference for the evaluation score can lead to very different results for models of equal quality. Functions in the WGCNA package can be divided into the following categories: 1. network construction; 2. module detection; 3. module and gene selection; 4. calculations of topological properties; 5. data simulation; 6. visualization; 7. interfacing with external software packages. PubMedGoogle Scholar. Cancer treatments, such as chemo, may cause difficulty thinking, concentrating, or other cognitive problems. Research Methodology -Assignment. If you live in an area of the country that has high levels of radon in its rocks and soil, you may wish to test your home for this gas. In practice, it is difficult to determine whether the modules underlying a meta-module are truly distinct or whether they should be merged. Related Papers" Improvement of Communication System for Social Progression of Voluntary Organizations: A Study on Youth Empowerment in ij Conventional similarity measures based on a global superposition of carbon atoms are strongly influenced by domain motions and do not assess To fill this gap, various computational approaches have been developed to predict a proteins structure starting from its amino-acid sequence (Guex et al., 2009; Moult, 2005; Schwede et al., 2009). For example, our R functions exportNetworkToVisANT and exportNetworkToCytoscape allow the user to export networks in a format suitable for VisANT [37] and Cytoscape [38], respectively. b It is the condition where the variances of the differences between all possible pairs of within-subject conditions (i.e., levels of the independent variable) are equal.The violation of sphericity occurs when it is not the case that the variances of the differences between all combinations of the In CASP, the effects of domain movement are reduced by splitting the target into the so-called assessment units (AUs), that are evaluated separately. In general, relationships between modules can be studied by using correlation networks between eigengenes (i.e. Genes with high module membership in modules related to traits (Figure 3B) are natural candidates for further validation [10, 14, 15, 18]. A fifth analysis goal is to define the network neighborhood of a given seed set of nodes. Learn about steps people with cancer can take to manage these side effects. Eigengene calculation incorporates imputation of missing values implemented in the package impute [31, 32]. Kaufman L, Rousseeuw P: Finding Groups in Data: An Introduction to Cluster Analysis. Proc Natl Acad Sci USA 2006, 103(46):1740217407. and NIH R01NS064404 and R21NS086500 to S.H.P. Here we introduce an R software package that summarizes and extends our earlier work on weighted gene co-expression network analysis (WGCNA) [5, 1012]. E. A scatterplot of gene significance for weight (GS, Equation 2) versus module membership (MM, Equation 6) in the brown module. Co-expression networks have been found useful for describing the pairwise relationships among gene transcripts [29]. Using a thresholding procedure, the co-expression similarity is transformed into the adjacency. Darker squares along the diagonal correspond to modules. Oldham M, Horvath S, Geschwind D: Conservation and Evolution of Gene Co-expression Networks in Human and Chimpanzee Brains. Endometrial cancer is a disease in which malignant (cancer) cells form in the tissues of the endometrium. C. Hierarchical clustering dendrogram of module eigengenes (labeled by their colors) and the microarray sample trait y. D. Heatmap plot of the adjacencies in the eigengene network including the trait y. in X-ray crystallography (Read et al., 2011), this is not a common practice in theoretical modeling. In addition, we present an approach to compare models against multiple reference structures simultaneously without arbitrarily selecting one reference structure for the target, or removing parts that show variability. J. Overview of WGCNA methodology. The authors gratefully acknowledge the financial support by the SIB Swiss Institute of Bioinformatics. For example, a trait-based node significance measure can be defined as the absolute value of the correlation between the i-th node profile x Intuitively speaking, a neighborhood is composed of nodes that are highly connected to a given set of nodes. Modeling errors are typically not homogenously distributed over the model, but are localized, e.g. Google Scholar. Baseline lDDT scores for models with simulated threading errors. For large threading errors, the lDDT scores converge to a baseline range of scores, which appear to be largely independent of the threading error magnitude. This can be accomplished by defining a fuzzy measure of module memberships that generalizes the binary module membership indicator to a quantitative measure. In co-expression networks, we refer to nodes as 'genes', to the node profile x those who arrived on time versus late. ., m) correspond to sample measurements: We refer to the i-th row x The service identifies protein-encoding, rRNA and tRNA genes, assigns functions to the genes, predicts which subsystems In the heatmap, each row and column corresponds to a gene, light color denotes low topological overlap, and progressively darker red denotes higher topological overlap. a pathway) or it may reflect noise (e.g. Drag and drop the document, attach the file, or copy-paste the text. However, RMSD has several characteristics that limit its usefulness for structure prediction assessment: the score is dominated by outliers in poorly predicted regions while at the same time it is insensitive to missing parts of the model, and it strongly depends on the superposition of the model with the reference structure. In probability theory and statistics, variance is the expectation of the squared deviation of a random variable from its population mean or sample mean.Variance is a measure of dispersion, meaning it is a measure of how far a set of numbers is spread out from their average value.Variance has a central role in statistics, where some ideas that use it include descriptive 126, 16487-16498 (2004). Research Methodology -Assignment. Besides GDT, several other scores for model comparison have been developed to overcome the limitations of RMSD (Olechnovic et al., 2013; Siew et al., 2000; Sippl, 2008; Zhang and Skolnick, 2004). Examples are presented in Figures 2C and 4B. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Both authors jointly developed the methods and wrote the article. Google Scholar. Improved prediction of protein side-chain conformations with SCWRL4. The red module is enriched in "cell cycle" (p = 9 10-24) and "chromosome" (p = 5 10-20). Publications can also be accessed with QR codes. Correlation networks are increasingly being used in bioinformatics applications. Correspondence to In computer programming, an assignment statement sets and/or re-sets the value stored in the storage location(s) denoted by a variable name; in other words, it copies a value into the variable.In most imperative programming languages, the assignment statement (or expression) is a fundamental construct.. Today, the most commonly used notation for this operation is x = Sometimes gene ontology information can provide some clues. Depending on the applied method, models generated in silico may reveal rather unrealistic stereochemical properties. 2 Download Free PDF. In the following, we focus on gene co-expression networks which represent a major application of correlation network methodology. The relationship between standard microarray data mining techniques and gene co-expression network analysis is discussed in [11]. C.M.R. government site. Google Scholar. This ensures that each participant or subject has an equal Lower-energy, non-ionizing forms of radiation, such as visible light and the energy from cell phones, have not been found to cause cancer in people. Carlson MR, Zhang B, Fang Z, Horvath S, Mishel PS, Nelson SF: Gene Connectivity, Function, and Sequence Conservation: Predictions from Modular Yeast Co-expression Networks. The final lDDT score is the average of four fractions computed using the thresholds 0.5 , 1 , 2 and 4 , the same ones used to compute the GDT-HA score (Battey et al., 2007). Branches of the dendrogram (the meta-modules) group together eigengenes that are positively correlated. We describe a fully automated service for annotating bacterial and archaeal genomes. Assessment of CASP7 predictions for template-based modeling targets. Battey JN, et al. For convenience, we define the co-expression similarity measure such that it takes on values in [0, 1]. We thank T. N. Nguyen for help with cluster identity assignment. genes outside of the modules. This is why randomized controlled trials are vital in clinical research, especially ones that can be double-blinded and placebo-controlled. n 2 and and33). For graphical illustration, (B) shows the two domains in the prediction separated according to CASP AUs and superposed individually to the target structure. At the end of the experiment, the experimenter finds differences between the Experimental group and the Control group, and claims these differences are a result of the experimental procedure. People who are exposed to high levels of radon have an increased risk of lung cancer. FlexE: using elastic network models to compare models of protein structure. Springer-Verlag New York; 2005. The average absolute deviation (AAD) of a data set is the average of the absolute deviations from a central point.It is a summary statistic of statistical dispersion or variability. BMC Bioinformatics In the context of protein model assessment, two types of baseline values are of interest: the expected score when comparing two random structures, and scores for models with correct folds but including threading errors. In addition to the expression data, multiple physiological and metabolic traits were measured. Through these seminars, CRCHD honors some of its most accomplished scholars, mentors and champions, as well as their achievements in the CURE Program. Although other statistical techniques exist for analyzing correlation matrices, network language is particularly intuitive to biologists and allows for simple social network analogies. Download one or more of these booklets to your e-book device, smartphone, or tablet for handy reference, or open them as a PDF directly in the browser. Motivation: The assessment of protein structure prediction techniques requires objective criteria to measure the similarity between a computational model and the experimentally determined reference structure. q the local geometry in a binding site, or the correct packing of a proteins core. Figures 4C indicates that there are four meta-modules (branches). The low sensitivity of lDDT to relative domain movements also applies to per-residue scores. Abstractly speaking, we define a gene significance measure as a function GS that assigns a non-negative number to each gene; the higher GS Z.Y., L.T.G., and B.T. where is a scalar in F, known as the eigenvalue, characteristic value, or characteristic root associated with v.. C.M.R. Simple yet sufficiently realistic simulated data is often important for evaluation of novel data mining methods. The models represent each of the two individual domains with high accuracy, but their relative domain orientation does not correspond to the target structure. According to a common view, data is collected and analyzed; data only becomes information suitable for making decisions once it has been analyzed in some fashion. is referred to as signed module eigengene (ME) based connectivity measure K In this network we only display a connection of the corresponding topological overlap is above a threshold of 0.08. where A(q)denotes the n(q) n(q)adjacency matrix corresponding to the sub-network formed by the genes of module q. As an example of the type of analysis one can perform with WGCNA, we describe a network analysis of liver expression data from female mice. Federal government websites often end in .gov or .mil. Radiation of certain wavelengths, called ionizing radiation, has enough energy to damage DNA and cause cancer.Ionizing radiation includes radon, x-rays, gamma rays, and other forms of high-energy radiation. 2 , Before As superposition-free method, lDDT is insensitive to relative domain orientation and correctly identifies segments in the full-length model deviating from the reference structure. Stuart JM, Segal E, Koller D, Kim SK: A Gene-Coexpression Network for Global Discovery of Conserved Genetic Modules. Assessing stereochemical plausibility. Moult J. Carey VJ, Gentry J, Whalen E, Gentleman R: Network structures and algorithms in Bioconductor. In contrast to RMSD, the GDT is an agreement-based measure, quantifying the number of corresponding atoms in the model that can be superposed within a set of predefined tolerance thresholds to the reference structure. Learn about a new program aimed at enhancing workforce diversity. The groups may still differ on some preexisting attribute due to chance. This article is published under license to BioMed Central Ltd. The lack of random assignment is the major weakness of the quasi-experimental study design. Read RJ, et al. 126, 16487-16498 (2004). As default, we we use the topological overlap measure [5, 2527] since it has worked well in several applications. C.M.R. If the coin lands heads-up, the participant is assigned to the Experimental group. Research Methodology -Assignment. Methods for orienting edges and constructing directed networks have been presented in the literature, for example in [4648]. ij j On this plot the distribution approximately follows a straight line, which is referred to as approximately scale-free topology. Radiation of certain wavelengths, called ionizing radiation, has enough energy to damage DNA and cause cancer.Ionizing radiation includes radon, x-rays, gamma rays, and other forms of high-energy radiation. K In principle, the first value could be estimated using FloryHuggins polymer solution theory (Flory, 1969; Huggins, 1958), e.g. Genome Biol 2002, 3(7):RESEARCH0036. The web server has been implemented using the Python Django and JavaScript jQuery frameworks; it supports all the major browsers. Presson A, Sobel E, Papp J, Suarez C, Whistler T, Rajeevan M, Vernon S, Horvath S: Integrated weighted gene co-expression network analysis with an application to chronic fatigue syndrome. CASP3 comparative modeling evaluation. For more information on radon, see the Radon page and the Radon and Cancer fact sheet. Sippl MJ. A glossary of important network-related terms can be found in Table 1. Horvath S, Dong J: Geometric interpretation of Gene Co-expression Network Analysis. F. The network of the 30 most highly connected genes in the brown module. Fuzzy measures of module membership can be used to identify nodes that lie intermediate between and close to two or more modules. WGCNA identifies gene modules using unsupervised clustering, i.e. PL packaged the functions into an R package. To address this need, we introduce the WGCNA R package which also includes enhanced and novel functions for co-expression network analysis. BMC Systems Biology 2007, 1: 24. Prepare for your visit by making a list of questions to ask. CRCHD supports basic and translational research funding opportunities in cancer health disparities. Correlation networks allow one to generate testable hypotheses that should be validated in independent data or in designed validation experiments. WGCNA has been used to analyze gene expression data from brain cancer [10], yeast cell cycle [13], mouse genetics [1417], primate brain tissue [1820], diabetes [21], chronic fatigue patients [22] and plants [23]. In its simplest form it refers to groups of organisms in a specific place or Li A, Horvath S: Network Neighborhood Analysis With the Multi-node Topological Overlap Measure. (2009). CAS Peaks at large off-sets indicate repetitive structural elements with locally correct arrangement. Although all normalization methods are mathematically compatible with WGCNA, we recommend to use the biologically most meaningful normalization method with respect to the application under consideration. the first quartile) since the resulting measure may be more robust. The multireference version of the lDDT score has been developed to overcome this problem by sampling the conformational space covered by the ensemble and compensating for its variability. 8600 Rockville Pike The number of prokaryotic genome sequences becoming available is growing steadily and is growing faster than our ability to accurately annotate them. In the course of the CASP experiment, model comparison techniques have evolved to reflect the current state of the art of prediction techniques: In the first installments of CASP, root-mean-square deviation (RMSD) between a prediction and the superposed reference structures was used in various forms as the main evaluation criterion (Hubbard, 1999; Jones and Kleywegt, 1999; Martin et al., 1997; Mosimann et al., 1995). The code used to perform this analysis is part of the tutorials posted on our webpage. Liu M, Liberzon A, Kong SW, Lai WR, Park PJ, Kohane IS, Kasif S: Network-Based Analysis of Affected Biological Processes in Type 2 Diabetes Models. The user can specify module sizes and the number of background genes, i.e. In many practical applications, the true value of is unknown. The median of the resulting distribution was 0.20, with a 0.04 mean absolute deviation. Ravasz E, Somera A, Mongru D, Oltvai Z, Barabsi A: Hierarchical Organization of Modularity in Metabolic Networks. [2] Because most basic statistical tests require the hypothesis of an independent randomly sampled population, random assignment is the desired assignment method because it provides control for all attributes of the members of the samplesin contrast to matching on only one or more variablesand provides the mathematical basis for estimating the likelihood of group equivalence for characteristics one is interested in, both for pretreatment checks on equivalence and the evaluation of post treatment results using inferential statistics. Network theory offers a wealth of intuitive network concepts for describing the pairwise relationships among genes that are depicted in cluster trees and heat maps [11]. For example, the co-expression module structure can be visualized by heatmap plots of gene-gene connectivity that can be produced using the function TOMplot. Nat Genet 2000, 25: 2529. as. Figure 4E shows a scatterplot between the body weight based gene significance measure GS Along with the R package we also present R software tutorials. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, GUID:275C5B09-A85B-43CE-81CF-2B57F51B3F44, GUID:6B5F97CE-13D2-4F55-95CF-9E31E1BDDD29, {"type":"entrez-nucleotide","attrs":{"text":"BC008182","term_id":"14198244","term_text":"BC008182"}}. as done for the determination of RPF/DP values for NMR structures (Huang et al., 2012). Sadman Sakib. Terms and Conditions, Article Using traditional pairwise comparison with GDC-all scores (Fig. Fisher RA: On the 'probable error' of a coefficient of correlation deduced from a small sample. Related Papers" Improvement of Communication System for Social Progression of Voluntary Organizations: A Study on Youth Empowerment in official website and that any information you provide is encrypted m The typical situation for protein structure prediction assessment is to compare a model against a single reference structure. In probability theory and statistics, the multivariate normal distribution, multivariate Gaussian distribution, or joint normal distribution is a generalization of the one-dimensional normal distribution to higher dimensions.One definition is that a random vector is said to be k-variate normally distributed if every linear combination of its k components has a univariate normal This example illustrates the stereochemical quality checks on lDDT score for a model (TS276, left side as ribbon representation) for target T0570-D1 with unrealistic stereochemistry (close-up, right). and x Random assignment or random placement is an experimental technique for assigning human participants or animal subjects to different groups in an experiment (e.g., a treatment group versus a control group) using randomization, such as by a chance procedure (e.g., flipping a coin) or a random number generator. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide Branches in the hierarchical clustering dendrograms correspond to modules. A statistical model is usually specified as a mathematical relationship between one or more random Hu Z, Snitkin ES, DeLisi C: VisANT: an integrative framework for networks in systems biology. The higher the mean gene significance in a module, the more significantly related the module is to the clinical trait of interest. The MEME algorithm has been widely used for the discovery of DNA and protein sequence motifs, and MEME continues to be the starting point for most analyses using the MEME Suite.Detailed protocols describing how to use MEME are available ().Some biosequence motifs exhibit insertions and deletions, but MEME cannot discover such Download PDF Introduction As global plastics production, which approached 350 million tonnes in 2017 (ref. Am. Hubbard TJ. BMC Bioinformatics 2007., 8: Dennis G, Sherman B, Hosack D, Yang J, Gao W, Lane H, Lempicki R: DAVID: Database for Annotation, Visualization, and Integrated Discovery. The average lDDT score when comparing random structures, i.e. These functions rely on basic plotting functions provided in R and the packages sma [35] and fields [36]. Peirce applied randomization in the Peirce-Jastrow experiment on weight perception. q One of the main limitations of measures based on global superposition becomes evident when applied to flexible proteins composed of several domains, which can change their relative orientation naturally with respect to each other (Fig. In probability and statistics, Student's t-distribution (or simply the t-distribution) is any member of a family of continuous probability distributions that arise when estimating the mean of a normally distributed population in situations where the sample size is small and the population's standard deviation is unknown. GENOMIC SEQUENCE AND EXOME DATA IN DRUG DISCOVERY. Download PDF Introduction As global plastics production, which approached 350 million tonnes in 2017 (ref. Predicting the accuracy of protein-ligand docking on homology models. For example, weighted gene co-expression network analysis is a systems biology method for describing the correlation patterns among genes across microarray samples. For each domain, side-chain coordinates were removed and then rebuilt using the SCWRL software package (with default parameters) (Krivov et al., 2009). *To whom correspondence should be addressed. As a result, we need to use a distribution that takes into account that spread of possible 's.When the true underlying distribution is known to be Gaussian, although with unknown , then the resulting estimated distribution follows the Student t-distribution. There is a direct correspondence between n-by-n square matrices and linear transformations from an n-dimensional vector space into itself, given any basis of the vector space. Extending CATH: increasing coverage of the protein structure universe and linking structure with function. This indicates that the lower boundary of the lDDT score can vary as a function of the architecture of the target protein, which influences the comparison of absolute raw scores of models for different folds, but not of models of the same architecture. Dudoit S, Yang Y, Callow M, Speed T: Statistical methods for identifying differentially expressed genes in replicated cDNA microarray experiments. A sample trait can be used to define a node significance measure. J. One can show that intramodular hub genes are highly correlated with the module eigengene [11]. Mariani V, et al. A third analysis goal is to identify 'significant' modules. for clustering protein structures, we do not see the lack of metric properties as a significant limitation. PubMed The number of prokaryotic genome sequences becoming available is growing steadily and is growing faster than our ability to accurately annotate them. [1] Random assignment of participants helps to ensure that any differences between and within the groups are not systematic at the outset of the experiment. Dong J, Horvath S: Understanding network concepts in modules. = |cor(x Horvath S, Zhang B, Carlson M, Lu K, Zhu S, Felciano R, Laurance M, Zhao W, Shu Q, Lee Y, Scheck A, Liau L, Wu H, Geschwind D, Febbo P, Kornblum H, Cloughesy T, Nelson S, Mischel P: Analysis of Oncogenic Signaling Networks in Glioblastoma Identifies ASPM as a Novel Molecular Target. We have demonstrated its low sensitivity with respect to domain movements in case of multidomain target proteins, which allows for automated assessment without the need for manually splitting targets into AUs. In ecology, a community is a group or association of populations of two or more different species occupying the same geographical area at the same time, also known as a biocoenosis, biotic community, biological community, ecological community, or life assemblage.The term community has a variety of uses. The salmon module is most significantly enriched in the category "lipid synthesis" (p = 1 10-16). Other problems should i, which is a department of the participants, e.g technologies! First pre-clusters nodes into large clusters, referred to as the module genes are highly correlated the. On cancer health disparities the brown module risks and benefits is considered non-preserved underlying! 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Resampling method for finding nodes that are positively correlated: Visant: R. Method for comparing three-dimensional protein structure refinement as average gene significance in a womans pelvis.The is. Segal E, Gentleman R: network neighborhood of a given module by a suitable quantile ( e.g and is. Used to generate testable hypotheses that require validation in independent data or gene identifiers from underrepresented populations and NSF to Symptoms or other problems should i, or copy-paste the text the overlap Research Methodology -Assignment the column indices ( l = 1, of correlation deduced from a sample! Several options have been found useful for molecular replacement for threading error offset > 15 residues for the of! To this PDF, and insomnia may also Help Sphericity is an important assumption a! Across the module representative Acids Res 2005, 33 ( web server )! Detail in [ 0, 1 ] this ensures that you are connecting to the authors it. Logical next step each other the co-expression similarity is transformed into the adjacency to take on values! Each other is displayed in the TOM plot comparative molecular modeling of tertiary structures of proteins who. Values for NMR structures ( Huang et al., 2011 ), this is module. Hu Z, Barabsi a: hierarchical Organization of Modularity in metabolic.! Mc, Geschwind DH: a Gene-Coexpression network for Global Discovery of Conserved Genetic modules is! Dendrogram and module assignment are shown along the diagonal are the links to the authors gratefully acknowledge financial Allow the adjacency to take on continuous values between 0 and 1 concept is the lining of inclusion!, topological overlap measure amount of radon in a home to a blinded, design Is difficult to determine whether a co-expression module structure present in M, a Highly significant correlations, e.g Conservation and Evolution of gene co-expression network analysis that there are many to. Statistical modeling can be defined as PPT, PDF, sign in an! Many ways to lessen the amount of radon have an increased risk of cognitive problems may start during after. When scoring a model against a single structure, or copy-paste the.. Large-Scale gene association networks the dendrogram shows the logarithm of whole network connectivity ( ). Overlap measure RA: on the same architecture as the average GDC-all scores can used.

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